The accelerated oxidation reaction was carried out on an oxidation simulator to study the oxidative stability of methyl oleate.The properties of the oxidative oils were analyzed such as peroxide value,acid value and kinematic viscosity(40 ℃),and the oxidation rates were explored under the condition of different temperature and oxygen flow rate.The oxidation process of methyl oleate was further characterized through Fourier transform infrared spectroscopy,and the induction period of methyl oleate was determined by the Rancimat method.The oxidation mechanism of oil sample was further studied through determining the reaction activation energy.The results show that the oxygen flow rate has less influence on the oxidation stability of oil sample,but the induction period is decreased and the oxidation stability of oil sample is obviously decreased with the increase of temperature.The double-bond isomerization transformation might be occurred in methyl oleate molecule during the oxidation of test sample according the test result of FTIR.The activation energy of oleic acid methyl ester obtained from Arrhenius equation is 34.058 kJ/mol.