Aiming at the unique climbing and multi terrain adaptability of gecko,the biological adhesion array of the animal was simulated by means of negative pressure control.On the basis of vacuum pressure analysis and adsorption force calculation,the two equation turbulence model in standard k-ε form was applied to the numerical simulation of negative pressure inside the suction cup.A non-structural fluid calculation domain with single-phase and steady-state was constructed by ICEM CFD and FLUENT software,and pressure-velocity coupling solving and analysis of the flow field was carried out through SIMPLE algorithm.It is found that there is obvious turbulent in the flow field,the dynamic pressure (0.154 MPa) and flow velocity (503 m/s) in outlet pipeline are much larger than those in inner field,and the central velocity in the outlet area is about 4.8 times of that in the inlet area,which is helpful to the air flow vacuumization and formation of the negative pressure.The results of finite element static analysis show that there is local stress concentration (>180 MPa) at bottom edge of the wall on suction cup,and the thickness of this area should be increase appropriately.The feasibility of adsorption mechanism with negative pressure and its realization method is verified by performance test of bionic prototype,and the experimental results of adsorption and mobile meet the expectation of flow field simulation.The study provides valuable support for bionic research and practical application of gecko adhesion array.