Rubber-plastic sealing structure is the key component of small-flow charging pump in nuclear power plant, and its wear life is directly related to the service performance and service life of the whole equipment. The life isokinetic test cycle of sealing structure is long and consumes manpower and material resources, so it is particularly important to adopt accelerated test method. In this paper, the accelerated test method for life evaluation of V-shaped rubber-plastic reciprocating seal structure is studied. Under the condition of ensuring the consistency of seal structure failure law, the accelerated test scheme is designed by changing seal failure factors. The reciprocating seal test platform was built to obtain the accelerated factor of seal life, and the feasibility of the accelerated test scheme was verified. The results show that the acceleration factor of seal life is 7.29, and the error with the predetermined acceleration factor is 8.88%, which verifies the feasibility and accuracy of the acceleration scheme and lays the foundation for the high reliability and industrial production of rubber-plastic seals.